Dual targeting of Myxococcus xanthus protoporphyrinogen oxidase into chloroplasts and mitochondria and high level oxyfluorfen resistance
نویسندگان
چکیده
منابع مشابه
Characterisation of the flavin adenine dinucleotide binding region of Myxococcus xanthus protoporphyrinogen oxidase
Protoporphyrinogen oxidase (PPOX), the penultimate enzyme in the haem biosynthetic pathway catalysers the six electron oxidation of protoporphyrinogen-IX to protoporphyrin-IX, in the presence of flavin adenine dinucleotide (FAD) and oxygen. In humans, partial defects in PPOX result in variegate porphyria. In this study, the FAD binding region in Myxococcus xanthus PPOX was analysed by engineeri...
متن کاملChloramphenicol resistance in Myxococcus xanthus.
Derivatives of Myxococcus xanthus FB(t) resistant to chloramphenicol (25 mug/ml) arose spontaneously with a frequency of approximately 10(-7). One of these organisms (FB(t)Cam(1) (r)) was characterized. FB(t)Cam(1) (r) showed a unique type of phenotypic instability. After transfer from medium containing chloramphenicol to medium lacking the drug, resistance was lost after approximately one gene...
متن کاملDual Targeting of Glutathione Reductase to Mitochondria and Chloroplasts
..................................................................................................................................... 4 List of Publications for the thesis............................................................................................. 5 Abbreviations .......................................................................................................................
متن کاملMitochondrial targeting of normal and mutant protoporphyrinogen oxidase.
We have investigated the signal sequence for mitochondrial transport of mutants (I12T, 78insC, IVS2-2a-->c, 338G-->C, R152C, 470A-->C, and L401F) and the wild type protoporphyrinogen oxidase (PPOX), which is the penultimate enzyme in the heme biosynthesis. We constructed the corresponding green fluorescent protein fusion proteins and studied their intracellular localization in COS-1 cells. We s...
متن کاملTransport of proteins into mitochondria and chloroplasts
KEY WORDS post-translational transport precursor processing transit peptide organelle envelope. envelope receptors I. INTRODUCTION It has been well established that mitochondria and chloroplasts are not autonomous organeUes. These organelles are capable of nucleic acid and protein synthesis, but many soluble and membrane proteins that become localized in them are initially synthesized on cytopl...
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ژورنال
عنوان ژورنال: Plant, Cell & Environment
سال: 2004
ISSN: 0140-7791,1365-3040
DOI: 10.1111/j.1365-3040.2004.01247.x